Graphpad plasma serial dilution4/5/2023 The card is gently placed on an automatic rotator and rotated continuously at 100 ± 2 rpm for 8 minutes.Once spread smoothly, a drop of diluted antigen suspension is added to the measured volume of specimen, positive and negative controls.One drop (50 µl) of the test specimen, positive and negative controls is pipetted onto separate reaction circles of the card.Those tests that are reactive by qualitative test are subjected to quantitative test to determine the antibody titres. The test can be performed both qualitatively and quantitatively. The tester antigen is prepared from a modified Venereal Disease Research Laboratory (VDRL) antigen suspension containing choline chloride to eliminate the need to heat inactivate serum, ethylenediaminetetraacetic acid (EDTA) to enhance the stability of the suspension, and finely divided charcoal particles as a visualizing agent.These antibodies are traditionally referred to as ‘reagins’. pallidum and possibly cardiolipin released from the treponemes. The test does not look for antibodies against the causative bacterium itself but rather for antibodies IgM and IgG against lipoprotein-like material released from damaged host cells caused by T.It is a rapid non-treponemal test that looks for non-specific antibodies in the blood of the patient that may indicate a syphilis infection.Rapid Plasma Reagin (RPR) like the Venereal Disease Research Laboratory (VDRL) testis a screening test for syphilis which is a sexually transmitted infection caused by the spirochete bacterium Treponema pallidum.
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